Flp-in cho
WebFRT site in the Flp-In™-CHO™ cell line has not been mapped, but has been demonstrated to have integrated into a highly transcriptionally active genomic locus as determined by … WebSeveral Flp-In™ host cell lines which stably express the lacZ-Zeocin™ fusion gene and contain a single integrated FRT site are available (see page 7 for ordering information). To express your gene of interest in 293, CV-1, CHO, 3T3, BHK, or Jurkat cells, you may want to use one of the Flp-In™ host cell lines to establish
Flp-in cho
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WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … WebAug 8, 2016 · MIL75-pFRT-FTMK and POG44 were co-transfected (1:9) into Flp-In-CHO cells. After 48 h of transfection, the cells were cultured under selection pressure by hygromycin B (1 mg/ml).
WebJul 17, 2024 · In order to test the Flp-in tm-ready cells, Lipofectamine 2000 was used to transfect 5 × 10 4 Lec 3.2.8.1 or 4 × 10 5 DG44 CHO cells with our GOI, in this case 100 ng of circular AQP3-GFP_pcDNA5/FRT … WebThe Flp-In™ System allows for stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp …
WebJan 21, 2011 · The transport assay for other sphingolipids was performed with Flp-In-CHO/SPHK1 cells stably expressing each transporter as described previously. The … WebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site
WebFeb 13, 2024 · Flp‐In CHO‐METKO cells (METKO), wild‐type MET (MET WT)–, or 11N all 11Q mutant MET (11N all 11Q)–transfected Flp‐In CHO‐METKO cells were stained with FITC‐conjugated anti‐MET antibody and analyzed by flow cytometry. C, Confocal microscopy analysis of MET (red) and nuclear (cyan). Flp‐In CHO‐METKO cells …
WebMar 5, 2024 · Idiopathic Basal Ganglia Calcification (IBGC) is a rare neuropsychiatric illness also known as Fahr's disease or Primary Familial Brain Calcification (PFBC). IBGC is caused by SLC20A2 variants, which encodes the inorganic phosphate (Pi) transporter PiT-2, a transmembrane protein associated with Pi homeostasis. the proper peacockWebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each the proper order to watch star warsWebDec 1, 2024 · Flp-In-CHO cells (Life Technologies) were maintained in F12 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37 °C in a humidified incubator containing 5% CO 2. The cells were transfected with 1 μg plasmid (pDF-λ1:pOG44 was 1:9) using Lipo 2000 (Life Technologies) according to the … signature\u0027s kirkland fancy mixed nuts 40 ozWebDiagnostic Tests. ANKLE/BRACHIAL INDEX. BLOOD PRESSURE TEST. CARDIAC CATHETERIZATION. CARDIAC STRESS TESTING. CAROTID ULTRASOUND. CORONARY CALCIUM SCAN. CORONARY COMPUTED TOMOGRAPHY ANGIOGRAPHY (CCTA) ECHOCARDIOGRAPHY. the proper peacock antiquesWebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them. the proper party companyWebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster … signature upholstery and design hartford ctWebObjective To construct a double transfected Flp-In TM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance … the proper pie company haines city