Fos-tta

Author: fniw

August undefined, 2024

Expression of ChR2-mCherry and c-Fos in c-fos-tTA/TRE

Web4 Apr 2024 · Tg (Fos-tTA)1Mmay. Mutation details : The c-fos-tTA transgene was designed with the promoter region from the mouse FBJ osteosarcoma oncogene (Fos or c-fos; at least 611 bp of 5' untranscribed sequence that encompasses the known mouse c-fos regulatory elements) placed upstream of the tetracycline-regulated transactivator gene … Web126 and TetTag-c-fos-tTA mice. TetTag-cFos-tTA mice were obtained from Jackson 127 Laboratory and crossed with C57BL/6J mice. Mice carrying the Fos-tTA transgene were 128 selected; Fos-tTA mice have a Fos promoter driving expression of nuclear-localized, 129 two-hour half-life EGFP (shEGFP) (Du et al., 2024; Koffman and Du, 2024; Ramirez et asas persamaan di depan hukum

MMRRC:031756-MU

WebpAV-c-Fos-tTA: Promoter: c-Fos: Tag: tTA: Serotype: AAV-DJ: Storage Buffer: PBS with 0.001% Pluronic F-68: Storage Conditions-80°C: Shipping: Dry ice: Availability: Made-to … WebtTA (tetracycline-controlled transactivator) (N terminal on insert) Fos 3' UTR (~1500bp) (N terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site … Web018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J These cfos-htTA mice (cfos-tTA/cfos-shEGFP) have two co-injected transgenes, cfos-tTA and cfos-shEGFP, resulting in … asas persamaan adalah

Optogenetic reactivation of memory ensembles in the ... - PNAS

Addgene: fos-tTA

Web23 May 2024 · Activity-dependent and Inducible Tagging of Dorsal and Ventral Hippocampus. We first infused an inducible, activity-dependent virus cocktail of AAV9-c-Fos-tTA and AAV9-TRE-eYFP into the dorsal or ventral dentate gyrus (DG) of adult male mice, which labeled DG neurons expressing the immediate-early gene c-Fos in a … WebAAV8-c-Fos-tTA What's in the box? Use Restrictions For Research Use Only. Not for Use in Therapeutic or Diagnostic Procedures. Storage Conditions -80°C Resources Downloads … asas persamaan kedudukanWebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies … asas persamaan derajat

"WebOne allele encodes the doxycycline sensitive tetracycline transactivator (tTA) under the control of the c-fos promoter. tTA binds the tetracycline operator (TetO) sequence to … " - Fos-tta

Fos-tta

Web21 Jan 2013 · (A) The tetracycline-transactivator (tTA) system for transgene regulation was combined with the c- fos promoter to tag activated neurons. In the absence of doxycycline (DOX), activation of the c- fos promoter leads to expression of an H2B-GFP fusion protein, which is stable for weeks after induction. Web4 Apr 2024 · Tg (Fos-tTA)1Mmay. Mutation details : The c-fos-tTA transgene was designed with the promoter region from the mouse FBJ osteosarcoma oncogene (Fos or c-fos; at …

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Web29 Oct 2014 · Finally, we show that crossing tetO-TRAP mice with transgenic Fos-tTA mice enables the expression of EGFP-L10a in CA1 pyramidal neurons that are activated … WebFos-tTA may only achieve workable signal-to-noise ratio (S/N) in brain regions with very low basal expression of Fos. Also, while reporter expression outlasts Fos protein, Fos-tTA cannot drive permanent effector expression, so manipulations are limited to a …

Web13 Mar 2024 · The c-Fos+ proportion among all tdTomato-labeled vCA1 neurons as well as c-Fos+ and tdTomato+ cell density was significantly higher in mice exposed to the same … WebThe rectangular area in a–l is magnified to demonstrate localization of ChR2-mCherry and c-Fos in representative neurons. m Number of DG granule neurons in sections near the injection site ...

Web2 Dec 2024 · 慢性吗啡治疗后，CRH CeA→VTA 神经元中c-Fos表达增加。 ... 小鼠VTA感染AAV-sgcrhr1-mcherry、RAM-tTA-TRE-Cre和Cre依赖性AAV-DIO-saCas9（Figure 6I）。经过光遗传学+行为学的验证，最终证明：CRH-CRHR1信号通路有助于抑制CeA输入和Mor-Ens回路的突触和功能重塑，从而调节阿片类药物 ... WebSubjects. c-fos-tTA animals were adult transgenic mice heterozygous for the transgene carrying the c-fos promoter-driven tetracycline transactivator (tTA) 21. Mice were bred …

Web5 Sep 2024 · We developed a technique that combines a head-mounted, miniature fluorescent microscope, with Thy1-G-CaMP7/c-fos-tTA double transgenic mice. The hippocampal CA1 region in double transgenic mice...

Web29 Oct 2014 · Finally, we show that crossing tetO-TRAP mice with transgenic Fos-tTA mice enables the expression of EGFP-L10a in CA1 pyramidal neurons that are activated during a fear conditioning trial. The tetO-TRAP mouse can be combined with other tTA mouse lines to enable gene expression profiling of a variety of different cell-types. asas persamaan hukum adalahWeb11 Sep 2024 · Neuronal-activity-induced tTA binds the tetracycline-response element, which in turn drives the expression of fluorescent proteins and other genetically encoded tools. … asas persamaan dalam hukumWeb23 May 2024 · (D and E) A virus cocktail of AAV9-c-Fos-tTA and AAV9-TRE-ChR2-eYFP (ChR2) was infused into the dorsal or ventral hippocampus, and AAV9-c-Fos-tTA and AAV9-TRE-hM4Di-eYFP (hM4Di) or AAV9-TRE-eYFP (eYFP) was infused into the basolateral amygdala (BLA). Mice were fear conditioned in context A while on Dox and in … asas persamaan dihadapan hukumWebThe rectangular area in a–l is magnified to demonstrate localization of ChR2-mCherry and c-Fos in representative neurons. m Number of DG granule neurons in sections near the … asas persidangan terbuka untuk umumWeb4 Jun 2024 · Although the c-Fos-tTA system enabled us to identify and manipulate engram cells, there was no approach to investigate their patterns of activity. When a neuron is activated, Ca 2+ flows into the soma. This neuronal activity can be translated into green fluorescence of a Ca 2+ indicator, such as GCaMP [ 17, 18 ]. asas persatuan dan kesatuanWeb3 May 2024 · Results. To label and optically reactivate a subpopulation of hippocampal DG granule neurons, which were activated during memory encoding, we stereotaxically injected the DG of c-fos-tTA transgenic mice with AAV 9-TRE-ChR2-mCherry and implanted the mice with optical fibers targeting the injection site.This approach restricts the expression … asas personal adalahWeb8 Jan 2024 · Fos-tTa and WT male mice were used for chemogenetics experiments (Experiment 3). All mice were housed under a 12 h light/dark cycle (lights on at 7:00 A.M.) at the maintained temperature of 21 ± 1°C and 50 ± 5% relative humidity. Mice used in the Fos-GFP expression time course experiment were ∼20–25 weeks old at the time of testing. asas personalitas hukum pidana