SpletIn the present study, 86 T. cruzi field stocks (68 isolated from humans with defined presentations of Chagas' disease and 18 from triatomines) derived from four Brazilian geographic areas were typed by the PCR assay based on the DNA sequences of the mini-exon and 24S␣ rRNA genes. These stocks were ordered into the two major T. cruzi lineages. Splet05. jan. 2006 · RNA isolation and reverse transcription-PCR. TC1 tumors from control and rofecoxib-treated mice were harvested 10 days after rofecoxib treatment and homogenized in TRIzol reagent (Invitrogen, Carlsbad, CA). RNA (3 μg) from each tumor was reverse transcribed using 0.5 μg oligo(dT) (Promega, Madison, WI), 10 mmol/L deoxynucleotide …
TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and …
SpletThe new Waverly TC1 Thermal Cycler (PCR Machine) delivers high performance and high throughput with easy programming. The TC1 is built with cutting-edge technology and … SpletAn expanded library of Tc1 insertions was screened as described previously (Zwaal et al., 1993). An insertion into the rbf-1 locus was identified using two rounds of PCR. Tc1-specific oligonucleotides were used as described previously (Zwaal et al., 1993). The rbf-1-specific oligonucleotides used were as follows: Rbf-H5, 5 -GA- cai-helper删除
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SpletThe Tcz1/Tcz2 primers resulted in an intense 200-basepair amplicon with DNA extracted from blood equivalent to 0.02 parasites per assay when used with a simple DNA … Splet05. avg. 2024 · the expr ession of cytoplasmic pCR TC1 (Figure S9A and S10A) and downregulated the leve ls of total. CR TC1 (Figure S9A, S10A, S11, and S 12), nuclear CR TC1 (Figure S9A, S10A, S11, and S12), and ... Splet24. avg. 2007 · We report here a novel function of TC1 in the regulation of heat shock response. TC1 is upregulated by cellular stresses to mediate heat shock response in … cna long term care insurance lawsuit