Phospho ire1 antibody

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August undefined, 2024

WebOur Anti-IRE1 (Ser724) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse IRE1 (Ser724) and is antigen affinity purified from pooled serum. It is great for use in WB. Western blot of mouse whole brain lysate showing specific labeling of the ~130 kDa IRE1 protein phosphorylated at ... WebAnti-IRE1 (phospho S724) antibody [1F12] See all IRE1 primary antibodies Description Rabbit monoclonal [1F12] to IRE1 (phospho S724) Host species Rabbit Tested applications Suitable for: ICC, WB more details Species …

IRE1 alpha [p Ser724] Antibody - BSA Free - Novus …

WebApr 12, 2024 · Phospho-IRS-1 (Ser307) Antibody detects endogenous IRS-1 only when phosphorylated at serine 307. This antibody does not cross-react with other related … WebSeparation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody. Cell Counting Kit-8 (CCK-8) A convenient and sensitive way for cell proliferation assay and cytotoxicity assay. SYBR Safe DNA Gel Stain Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. ... portsmouth electric scooter hire

Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody …

Webphospho ire1 alpha phospho-ire1 alpha p-ire1 alpha Background Unfolded protein response (UPR) signaling is a mechanism used by eukaryotic cells to deal with endoplasmic reticulum/ER stress: an accumulation of unfolded proteins in the ER lumen. WebBoster Bio Anti-Phospho-IRE1 (S724) ERN1 Rabbit Monoclonal Antibody catalog # P00683. Tested in WB applications. This antibody reacts with Human. WebAug 23, 2024 · HEC-1-A cells were infected with HSV-1 (strain HF) at multiplicity of infection (moi) = 1. (A) Total cellular protein was harvested at indicated time point p.i. (post infection) and subject to Western blot analysis using antibodies against total IRE1, gD-1 (Glycoprotein D) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase). The number under ... portsmouth ehe

ZIKV infection activates the IRE1-XBP1 and ATF6 pathways of …

Phospho-IRE1 alpha (Ser724) Antibody from Thermo Fisher Scientific

WebDescription When detecting phospho-IRE1 Alpha (Ser-724) using NB100-2323, it is recommended to normalize its band intensity/immunoreactivity with total-IRE1 alpha. NB100-2324 and NB110-59971 can be used for the detection of ... and total IRE1 alpha Antibody [NB100-2324SS] before purchasing 100ug full vials. Product Application Details WebIRE1 Antibody detects endogenous levels of total IRE1. RRID: AB_2841178 Cite Format: Affinity Biosciences Cat# DF7709, RRID:AB_2841178. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: opus flyin\u0027 highWebAnti-IRE1 alpha (phospho Ser724) antibody (GTX132808) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls Proteins & Peptides Slides Lysates Serums & Plasmas Reagents Research Kits Research Tools Back Cancer Tumor Markers Neuroscience Cell Biology Metabolism … portsmouth education partnership

"WebThe protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity … " - Phospho ire1 antibody

Phospho ire1 antibody

Phospho-IRE1 alpha (Ser724) Antibody from Thermo Fisher Scientific

WebDescription. IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. WebIRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein …

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WebAnti-phospho IRE-1 (Cat. No. ab48187; 1:2 ,500) antibody was procured from Abcam ( Cambridge, MA, USA). The dilution factors used for Western blotting May 1, 2024 Tae Jin Kim, Do Hyeon Pyun, Myeong Jun Kim, Ji Hoon Jeong, A.M. Abd El-Aty, Tae Woo Jung

WebMay 7, 2024 · We generated an anti-phospho-S729 antibody to investigate such phosphorylation. Compared with pharmacological ER stress inducers or Toll-like receptor ligands, the bacterial subtilase cytotoxin has an unusual capability in causing rapid and strong phosphorylation at S729 and triggering B cells to express spliced XBP1. WebMar 8, 2024 · Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free See all IRE1 primary antibodies Description Rabbit monoclonal [EPR5253] to IRE1 (phospho S724) - BSA and Azide free Host species Rabbit Tested applications Suitable for: Dot blot, WB more details Unsuitable for: Flow Cyt,ICC/IF or IHC-P Species reactivity Reacts with: Human …

WebIRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. WebHowever, accumulation of unfolded proteins results in dissociation of BIP from IRE1 and oligomerization of IRE1 accompanied by the upregulation of GRP78/BIP which is consistent with our results [53]. Activation of IRE1 initiates the recruitment of the TRAF2/ASK1 complex and activate the Jun-N-terminal kinase (JNK), one of the stress kinases [54].

WebRabbit polyclonal antibody to IRE1 (phospho Ser724). (A258133) Buy rabbit polyclonal antibody to IRE1 (A258133). Applications: WB, ICC/IF and more. Reactivity: Human, …

WebIRE1 alpha (phospho Ser724) antibody Cat No. GTX130378 Datasheet File See all IRE1 alpha products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications. Calculated MW 110 kDa. ( Note ) Positive Control HeLa (100 nM Calyculin A, 30 min) Predict Reactivity opus firmaWebWestern blot - Phospho-IRE1-S724 Rabbit mAb (AP1146) Western blot analysis of extracts of HeLa cells, using Phospho-IRE1-S724 antibody (AP1146) at 1:1000 dilution.Hela cells … opus financialWebSep 21, 2024 · The primary antibodies used in this study were as follows: XBP1 (Abcam, ab37152), ATF6 (Abcam, ab37149), phospho-IRE1 (Abcam, ab48187), ZIKV envelope protein (BioFront Technologies, BF-1176-56). Statistical analysis All experiments were reproducible and carried out in triplicate. portsmouth education associationWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) Research with confidence – consistent and … opus files openWebIRE1α (14C10) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding His963 of human IRE1α. Background The … portsmouth eicWebThis antibody does not react with monkey samples. Suggested positive control: Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells (20 µg). Background/Target Information IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein opus first ameripriseWebTo characterize our anti–phospho-S729 antibody, we treated naive XBP1-proficient and -deficient B cells with LPS for a course of 72 h, immunoprecipitated IRE1 using an anti–mouse IRE1 antibody, and showed that this antibody recognizes the 130-kD phospho-IRE1 protein produced by 48-h and 72-h LPS-stimulated XBP1-deficient B cells . To ... opus file player download free