Phospho-mtor antibody

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August undefined, 2024

WebAnti-mTOR Antibody detects level of phospho-mTOR (Thr2446) & has been published & validated for use in WB. Research Category Signaling Research Sub Category … WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ...

Anti-p-mTOR Antibody (59.Ser 2448) SCBT - Santa Cruz Biotechnology

WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. WebKey features and details Rabbit polyclonal to mTOR (phospho S2448) Suitable for: WB, IHC-P, ICC/IF Reacts with: Human Isotype: IgG You may also be interested in Primary Anti … small kitchen for office

PhosphoPair mTOR (Ser2448) Antibody Set, mTOR, 6H9B10; …

Webindicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. mTOR Antibodies Phospho-Specific mTOR Antibodies mTOR CRISPR Knockout, HDR and Nickase Knockout Plasmids mTOR CRISPR Activation Plasmids mTOR siRNA, shRNA Plasmid and shRNA Lentiviral Particles gene silencers WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit Synonym (s): FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1 eCl@ss: 32160702 NACRES: NA.41 Pricing and availability is not currently available. Properties biological source rabbit Quality Level 100 antibody form affinity purified immunoglobulin WebRabbit recombinant monoclonal mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free. Validated in WB, IHC, Dot and tested in Mouse, Human. high yellow racial term

Phospho-mTOR (Ser2448) Antibody, PE (12-9718-42)

Activation mTOR Pathway for WB Biocompare Antibody Review

WebDescription: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. … WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … small kitchen great room designsWebThis affinity purified antibody has been tested for use in immunohistochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa. Reactivity in other immunoassays is unknown. Purity/Specificity small kitchen for studio

"WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … " - Phospho-mtor antibody

Phospho-mtor antibody

mTOR Antibody SCBT - Santa Cruz Biotechnology

WebULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to … WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold

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WebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide … WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the …

WebAn antibody to the phosphorylated S6 ribosomal protein was used to stain the tumors, all high-grade sarcomas. Pretreatment biopsy or resection material was tested: the original tumor (n=6) or tumor recurrence/metastasis (n=14); either of these may have been after treatment with other agents. WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl …

WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. Webadult animals (c) Immunoprecipitation (IP) with mTOR antibody efficiently extracts mTOR complexes from prepared lysates. mTOR is highly enriched in the IP fraction but is absent in the supernatant and the wash fractions. Raptor is ... Phospho-mTOR (Ser-2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389),

WebSep 5, 2024 · p-mTOR Antibody (59.Ser 2448) is an IgG1 κ mouse monoclonal p-mTOR antibody that detects Ser 2448 phosphorylated mTOR of mouse, rat and human origin by …

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR426 (2)] to mTOR (phospho S2448) - BSA and Azide free. Suitable for: IHC-P, … small kitchen for basement apartmentWebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. high yellow skin colorWebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … high yellow sunglasses lensWebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 ... small kitchen furniture for 2Webadult animals (c) Immunoprecipitation (IP) with mTOR antibody efficiently extracts mTOR complexes from prepared lysates. mTOR is highly enriched in the IP fraction but is absent … high yellow white trash lisa pageWeb4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and … high yellow skin tone termWebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot. small kitchen garden windows