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Phospho-yap ser127 antibody

WebJan 17, 2024 · Phospho (Ser127) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein. Validations Biological Strategies … WebA huge increase of YAP1 protein and YAP phosphorylation at Serine 127 (p-YAP Ser127) in HD over LD was detected. We also observed that DUSP10 overexpressing CRC cell lines, HT29-DUSP10 and HCT116-DUSP10, showed higher levels of YAP1 and p-YAP Ser127 than EV cells in LD, that were further augmented by HD (Figure 2d, Figure S3d).

HTRF ® Total and Phospho-YAP (Ser127) Cellular Assays - Springer

WebApr 11, 2024 · After blocking with 5% nonfat milk in TBST, the membranes were incubated with primary antibodies, including anti-Phospho-YAP (Cell Signaling Technology, CST#13008), anti-YAP (Cell Signaling Technology, CST#14074), and anti-S1pr2 antibody (21180-1-AP, Proteintech), followed by HRP-linked secondary antibodies. ... Our results … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) Research with confidence – consistent and … list of central qld towns https://minimalobjective.com

Phospho-YAP (Ser127) (D9W2I) Rabbit mAb - Cell Signaling …

WebImmunofluorescence analysis of Hela cell. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). WebSep 23, 2016 · The protein expression of YAP and p-YAP (Ser127) in Panc-1 exposed to resveratrol and gemcitabine was evaluated by Western blot analysis. As shown in Figure 6B, resveratrol treatment up-regulated the level of p-YAP (Ser127), and the total level of YAP was significantly inhibited by resveratrol. However, gemcitabine had no effect on YAP … WebYAP1 Antibody (Phospho-Ser127) (OAAJ02615), Catalog OAAJ02615. Tested in WB, IHC applications. Reacts with Human, Mouse, Rat samples. Liquid. ... Phospho-YAP (Ser127) antibody detects endogenous levels of YAP only when phosphorylated at Serine 127: Application Info: WB: 1:500-1:2000 IHC: 1:50-1:200: Target Info. Gene Symbol: images of the wheel

Phospho-YAP (Ser127) Antibody from Cell Signaling Technology

Category:Phospho-YAP (Ser127) Antibody from Cell Signaling Technology

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Phospho-yap ser127 antibody

(4911) Phospho-YAP (Ser127) Antibody - CiteAb

WebJun 18, 2024 · The fixed cells were stained with an anti-YAP (1:400, sc-271134, Santa Cruz) antibody in 2% BSA for an hour at room temperature and then with Alexa Fluor ® 488-conjugated goat anti-mouse IgG as... WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. Antibodies are purified by …

Phospho-yap ser127 antibody

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WebWestern blot analysis of extracts from HeLa cells (lane 1) or YAP knock-out cells (lane 2) using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb #13008 (upper), and GAPDH (D6H11) XP ® Rabbit mAb #5174 (lower). The absence of signal in the YAP knock-out HeLa cells confirms specificity of the antibody for YAP. WebProduct Name: Phospho-YAP (Ser127) (D9W2I) Rabbit mAb Product Type: Monoclonal Antibody Species of Origin: Rabbit Lot Number: 6 Concentration: 549 µg/ml Approved Applications: •Immunohistochemistry (Paraffin) •Immunoprecipitation •Western Blotting Approval: Production: Valerie Goss Date: 22-December-2024

WebMonoclonal Antibody for studying YAP (Ser127) phosphate. Cited in 316 publications. Validated for WB, IP, IHC. Available in 2 sizes. Highly specific and rigorously validated in … WebNov 30, 2024 · YAP1 Antibody (63.7) is an IgG2a κ mouse monoclonal YAP antibody suitable for the detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA. YAP1 Antibody (63.7) is available as the non-conjugated anti-YAP antibody form.

WebPhospho-YAP1 (Ser127) Polyclonal Antibody Product Details Size 100 µL Species reactivity Human, Mouse, Non-human primate, Rat Host / Isotype Rabbit / IgG Class Polyclonal Type Antibody Conjugate Unconjugated Immunogen A synthesized peptide derived from human YAP around the phosphorylation site of Ser127. Form Liquid Concentration 1 mg/mL WebInvitrogen Anti-Phospho-YAP1 (Ser127) Polyclonal, Catalog # PA5-114675. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) …

WebRabbit Polyclonal YAP1 (phospho Ser127) antibody. Validated in WB. Tested in Human. United States (US) Country / Location Selection Product ... This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural ...

WebHere, we describe a novel approach to detect both the relative amount of total YAP (T-YAP assay) and the phosphorylation of the residue S127 of YAP (S127-P-YAP assay) using a HTRF ®-based method. This easy-to-run method can easily be miniaturized and allows for a high-throughput analysis in 96/384-well plate format, requiring less cellular ... list of central universities of indiaWebPhospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127. Species Reactivity; Human, Mouse, Rat; Product Property. Format; Liquid; Buffer; Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Storage; Shipped at 4°C. Upon delivery aliquot and store at -20°C. images of the wheat and taresWebYAP1 Antibody (Phospho-Ser127) (OAAJ02615), Catalog OAAJ02615. Tested in WB, IHC applications. Reacts with Human, Mouse, Rat samples. Liquid. ... Phospho-YAP (Ser127) … list of ceo stepping down 2021WebDec 19, 2024 · The level of phosphorylation of the residues serine 127 (S127) of YAP is generally assessed in cellular models, native tissues, and organs, as a marker of YAP activity and location, and is regulated by numerous partners. This phosphorylation event is classically detected using a western blot technical approach. list of central university of indiaWebPhospho-YAP1 (Ser127) Antibody (PA5-117322) in WB Western blot analysis of Phospho-YAP1 (Ser127) in Untreated (–) and treated (+) HeLa whole cell extracts (30 µg). Samples were separated with 10% SDS-PAGE and incubated in Phospho-YAP1 (Ser127) polyclonal antibody ( Product # PA5-117322) using a dilution of 1:5,000. Product Details list of centurylink compatible modemsWebPhospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127. This antibody may cross-react with phospho-TAZ (Ser89). … list of century 21 real estate agentsWebPhospho (Ser127) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. list of ceo of famous companies